Ampicillin belongs to the beta-lactam antibiotic family and it irreversibly inhibits transpeptidase to prevent cell wall synthesis in bacteria. Ampicillin has broad spectrum activity against most gram-positive and gram-negative bacteria and is often used for treating bacterial infections such as respiratory tract infections, urinary tract infections and meningitis. Nevertheless, it has some common side effects including rash, nausea, diarrhea, seizures and anaphylaxis. The traditional techniques/instruments (HPLC or GC-MS) for detecting beta-lactam antibiotics are expensive, laborious, and time-consuming. Immunoassay techniques, such as ELISA, are commonly preferred as simple, reliable and rapid methods. BioVision’s Ampicillin ELISA Kit is a competitive-based ELISA that can detect ampicillin in milk, urine, tissues and serum. It can detect and quantify broad range of ampicillin (1 – 625 ng/ml) within 90 minutes.