PEB is an extraction buffer for disruption and solubilisation of total protein from plant tissue and algal cells. The use of the anionic detergent LDS together with the recommended procedure (combination of sonication and freeze/thaw cycles) has been shown to increase the number of solubilised and non-degraded proteins when compared to other methods of cell disruption (see reference). The estimated hands-on time for the recommended procedure is 20-30 minutes for 1-2 samples. Expected yields will be 1.5-6 ug/ul total protein (recovered from standard procedure) depending on the starting material, e.g. its biological stage, homogenization method used (bead beater vs. sonication).
应用类型
免疫原
来源宿主
1 | PEB（4x）| 蛋白质提取缓冲液产品的来源宿主，可查看提供的英文产品说明书。
反应性
保存建议
Stable at RT for at least 1 month; short-term storage (6 month) at 4°C and long term storage (1 year or more) at -20°C
