His标签，也称为多组氨酸标签（polyhistidine），His6标签或hexa组氨酸标签，这是一种在转染的细胞中目标蛋白的C端或N端上由至少6个组氨酸残基链接上所组成的氨基酸序列。

His-Tag是目前用于纯化的融合标签中应用最广泛的特点：

a：标签较短可置于蛋白的N端或者C端

b：对蛋白的功能及下游应用影响小

c：免疫原性低，可以和多种标签连用

d：纯化方式非常简单方便

这些属性使His标签成为重组蛋白生产和分离的流行标签。然而，对于未纯化的样本，His标签蛋白的准确定量样品仍然很困难。

作为专业的生命科学医药原料供应商，Cell Biolabs中国区金牌代理，kok全站app官网登录-Kok全站官网登录 科技为您推荐：His-Tag 蛋白ELISA 试剂盒，这是一种基于竞争性ELISA测定法，专为快速检测和定量His标签蛋白样品（C 端或 N 端）而开发。

引用文献：

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